| Easysize | |
| EBM Scandinavia A/S | Central Denmark Region |
| Ecreo | Region of Southern Denmark |
| eCrimeLabs ApS | |
| EDB Lee ApS | Region of Southern Denmark |
| edbpriser.dk | Region Zealand |
| EDnord | North Jutland Region |
| Eduflow | |
| EET Group | Capital Region of Denmark |
| EG Danmark A/S | Central Denmark Region |
| EgnsINVEST Ejendomme Tyskland A/S | |
| EIVA A/S | Central Denmark Region |
| Ektos | |
| eKvator ApS | Capital Region of Denmark |
| El-supply | Capital Region of Denmark |
| Elbek & Vejrup | Central Denmark Region |
| Electricity Maps ApS | |
| Elfa Distrelec A/S | Central Denmark Region |
| Elfac A/S | Central Denmark Region |
| Ellab A/S | |
| elmah.io | |
| Eloomi A/S | |
| elsewhere ApS | |
| Emagine | |
| Embark Biotech ApS | |
| Embark Laboratories ApS | |
| Embrace-IT ApS | |
| EMD International A/S | North Jutland Region |
| Empact | |
| Emplate ApS | |
| Enablement Solutions | |
| Encodify A/S | |
| Enduro Genetics ApS | |
| Energi Data Service | |
| Energimidt | Central Denmark Region |
| Enetpulse | |
| Enggaard Grafik | Region of Southern Denmark |
| Engsig A/S | North Jutland Region |
| Enrginet | |
| Enterspeed A/S | |
| Entity Framework Playground | |
| Ento Labs ApS | |
| EPC Technology A/S | Central Denmark Region |
| Epista Life Science | |
| EPLAN | Capital Region of Denmark |
| Epoka A/S | North Jutland Region |
| Ergo Partner ApS | Region Zealand |
| Ergotel A/S | Region Zealand |
| Erhvervsakademi Aarhus | |
| Erhvervsakademi Dania | |