| Assist Danmark | Capital Region of Denmark |
| Astralis | |
| Astro.io ApS | |
| AT-Orbital ApS | Central Denmark Region |
| ATAK Advertising & Marketing ApS | Capital Region of Denmark |
| ATC Data ApS | Region Zealand |
| Atea A/S | Capital Region of Denmark |
| Atenzo Digital A/S | |
| Ateo | |
| ATIVE | Capital Region of Denmark |
| Atlant 3D | |
| ATMOS | Capital Region of Denmark |
| AtomGraph | Capital Region of Denmark |
| ATRIUM Partners A/S | |
| Attech Computer ApS | Central Denmark Region |
| Attention Solutions ApS | Capital Region of Denmark |
| ATZnet | Region of Southern Denmark |
| Audio-Visuelt Centrum A/S | Central Denmark Region |
| Auditdata | |
| Auto IT | |
| Automatic Syd A/S | |
| Automize | |
| AutoPilot | |
| AV Distribution A/S | Central Denmark Region |
| Av-Cables.dk ApS | Central Denmark Region |
| Aviation eLearning ApS | |
| Avilex Pharma | |
| Axacon | |
| Axhouse | Region Zealand |
| Azero A/S | Central Denmark Region |
| b.energy | |
| B14 ApS | |
| B5 Digital | |
| Backup112 ApS | Capital Region of Denmark |
| Bacthera Denmark A/S | |
| Bactolife A/S | |
| Bankdata | Region of Southern Denmark |
| Barma | |
| Barner | Capital Region of Denmark |
| Bavarian Nordic | |
| BaxData | Region of Southern Denmark |
| BB Data Teknik A/S | Capital Region of Denmark |
| Be My Eyes | |
| BEAS A/S | Central Denmark Region |
| Beep Analytics ApS | |
| BeeSite ApS | Region Zealand |
| beeSmart | |
| Befro ApS | Central Denmark Region |
| Bekey | |
| Bellcom | Region of Southern Denmark |