| EVENET | Madrid |
| Everex | Madrid |
| Evicertia, S.L.U. | Madrid |
| Evolucionapp | Cádiz |
| Evotec | Madrid |
| Excuse Me Captain | |
| Exea Comunicación | Navarre |
| Exerta.pro | Valencia |
| Exis Inversiones en Consultoría Informática y Tecnología, S.A. | Madrid |
| Explora Digital | Madrid |
| Expocom S.A. | Barcelona |
| ExpoNet | Madrid |
| extendedWorks | Biscay |
| Extra Software, S.A. | Madrid |
| Extrenet Consulting | Badajoz |
| Ezzing Solar | Madrid |
| F9-Soft, S.L. | Pontevedra |
| Fab Lab Barcelona | Barcelona |
| Fabertelecom | Salamanca |
| Facenote | Madrid |
| Facephi | Alicante |
| Factorial | Barcelona |
| Facultad Padre Ossó | |
| Faes Farma | |
| Fagor Electrónica, S.Coop. | Gipuzkoa |
| Fairhall | Cádiz |
| Fama Systems | Madrid |
| Fantastic Solutions, S.L. | Barcelona |
| Faraday Venture Partners | Madrid |
| Farmasoft Informática y Servicios, S.L. | Madrid |
| Fast PCB, S.L. | Gipuzkoa |
| FC Barcelona Innovation Hub | Barcelona |
| Febrer Software | Biscay |
| FEDIT | Madrid |
| Feelcapital EAFI, S.L. | Madrid |
| Fegemu Automatismos, S.L. | Gipuzkoa |
| Fernando Guillén | Balearic Islands |
| Ferrín Electrónica, S.L. | Barcelona |
| Fersoft | Córdoba |
| Fesia Technology, S.L. | Gipuzkoa |
| FI Boost ES | Barcelona |
| Fibercom | Zaragoza |
| Fibernet, S.L. | Madrid |
| Fibranet NSP, S.L. | Barcelona |
| Fides Capital | Madrid |
| Fihoca | Barcelona |
| FileHawk | Barcelona |
| Filmac Centre, S.L. | Valencia |
| Filnet Serveis i Comunicacions, S.L. | Barcelona |
| Fina Biotech | Madrid |