| Upperclick | Valencia |
| Uptech Sensing | Navarre |
| Uquifa | Barcelona |
| UQUIFA Group | Barcelona |
| Urbanitae | Madrid |
| UrbiCAD Architecture, S.L. | Valencia |
| Uriach | Barcelona |
| Uritec | Navarre |
| USACD System, S.L. | Barcelona |
| USB Total | Álava |
| User Informática, S.L. | Valencia |
| User-Experience | Biscay |
| Utopia.es | Badajoz |
| Uttopion | |
| V-Mann | A Coruña |
| V-Valley | Madrid |
| VA Creativos | Alicante |
| VADAVO Soluciones, S.L. | Valencia |
| vadeCity, S.L.U. | Barcelona |
| ValenciaSEO | Valencia |
| Valgetal and Matego | Zaragoza |
| Validated ID, S.L. | Barcelona |
| Valnera, Consultoría y Sistemas, S.L. | Cantabria |
| ValoraData | Barcelona |
| Vaxdyn, S.L. | Sevilla |
| Vaxnu | Cádiz |
| Vayabits | Barcelona |
| Vector 3 | Barcelona |
| Vector Aplicaciones Informáticas, S.L. | La Rioja |
| Vectoraula, S.L. | Madrid |
| Veiss Comunicación, S.L. | Álava |
| Velca | |
| Verbio Technologies, S.L. | Barcelona |
| Verbio Technologies, S.L. | Barcelona |
| Veridas | Navarre |
| Veridata, S.L. | Madrid |
| Veritas Intercontinental | Madrid |
| Vermut Platform, S.L. | Barcelona |
| Vermut Platform, S.L. | Barcelona |
| Verse | Barcelona |
| Vertical Green Agritech, S.L. | Madrid |
| Verticalia | Pontevedra |
| Vetoclock | Zaragoza |
| Vexlan Marketing, S.L.U. | Madrid |
| VGS Desarrollo e implantación web, S.L. | Zaragoza |
| Viaindice | Barcelona |
| Viaintermedia interactive | Madrid |
| VIAWEB.es | Madrid |
| VICOMTECH | |
| VidaApp | Madrid |