| 2DEngine | New York |
| 2GBHosting | North Carolina |
| 2K Medical Billing Systems | California |
| 2Morrow, Inc. | Washington |
| 2msolutions, Inc. | Texas |
| 2nd Address | California |
| 2nd Line Marketing | |
| 2nd Watch, Inc. | Washington |
| 2nd.MD | Texas |
| 2ndNature Software, Inc. | California |
| 2ndWave Software | Arizona |
| 2Novas | |
| 2pensmedia | Illinois |
| 2Plus2 Partners, Inc. | California |
| 2POINT, Inc. | |
| 2Rivers Media | |
| 2S Marketing, LLC | Nevada |
| 2SD Toxicology Services, LLC | North Carolina |
| 2Secure.biz | New York |
| 2see Technology, LLC | Pennsylvania |
| 2seventy Bio, Inc. | Massachusetts |
| 2talk, LLC | |
| 2ten Marketing | |
| 2the Max USA, Inc. | California |
| 2U, Inc. | Maryland |
| 2uTec, LLC | New York |
| 2W Technologies, Inc. | |
| 2X | Pennsylvania |
| 3 Advertising | |
| 3 Day Startup | Texas |
| 3 Gtms, LLC | Connecticut |
| 3 Media Web | Massachusetts |
| 3 Points Specialties, Inc. | Minnesota |
| 3 Rivers Capital | Pennsylvania |
| 3 Rivers Venture Fair | Pennsylvania |
| 3 Waves Agency | |
| 3-D ImageTek Corporation | California |
| 3-dB Networks | Colorado |
| 3-GIS, LLC | Alabama |
| 3.7 Designs | Michigan |
| 301 Computers | Maryland |
| 301 Digital Media, LLC | Tennessee |
| 301 Interactive Marketing | Kentucky |
| 301Consulting | |
| 302 Interactive, LLC | Florida |
| 303 Software, Inc. | Colorado |
| 305 Spin, Inc. | Missouri |
| 308 Systems, Inc. | Colorado |
| 309marketing | |
| 310 Creative | |