| Rubica Consultores | |
| Salamandra Centro Internacional de Entrenamiento | |
| Salva Health | |
| SamXLabs, S.A.S. | |
| Sancrisoft | |
| Savne | |
| Saycom de Colombia | Bogotá |
| SCITECH Science & Technology | |
| Scrino | |
| SEAQ Servicios, S.A.S. | Bogotá |
| Seed EM | |
| Seguridad Informática Colombiana | Bogotá |
| Sempli | Antioquia |
| SEO Colombia | |
| SEO Web Colombia | |
| Servicio Nacional de Aprendizaje | |
| Serviunix S.A. | Antioquia |
| Shareppy, S.A.S. | Santander |
| Sigma Studios | Bogotá |
| Símbolo Interactivo | Antioquia |
| Simian, S.A.S. | |
| Simphony Technologies | |
| Simpl | |
| Sinerware, S.A.S. | |
| Sinfa | Bogotá |
| SingleClick Solutions, S.A.S. | |
| Sirius | Bogotá |
| Sirius | |
| Sistemas Enlínea, S.A. | |
| Sistemas TGR | Bogotá |
| Sitios y Web | Bogotá |
| Six Degrees IT | |
| Skina Technologies, S.A.S. | Bogotá |
| Slabcode | |
| SM Digital | Bogotá |
| Social Colectivo | Bogotá |
| Sociedad Colombiana de Matemáticas | Bogotá |
| Sofka | |
| SoftCaribbean | |
| Softgic | |
| Softline, S.A. | |
| SoftManagement, S.A. | |
| Softnet S.A. | Valle del Cauca |
| Softtronics Solutions | |
| Software Associates | Bogotá |
| Software Colombia | |
| Software House | Bogotá |
| Software Quality Assurance, S.A. | |
| Sogyo | Bogotá |
| Solución Digital | |