| Emmemedia | Naples |
| Emmetek Informatica S.r.l. | Florence |
| Emotiva S.r.l. | |
| Enapter S.r.l. | |
| Endian | South Tyrol |
| Endostart S.r.l. | Florence |
| Endurance S.r.l. | Bologna |
| Enel S.p.A. | Rome |
| Enel X Italia S.r.l. | |
| Enerbrain S.r.l. | |
| Energy Dome S.p.A. | Milan |
| Enerpoint S.r.l. | Monza and Brianza |
| EnExSys S.r.l. | Bologna |
| Enforma Solutions | Rovigo |
| Engage Consulting | Ravenna |
| enGenome | Pavia |
| Engine Net S.a.s. | Genoa |
| Engineering Ingegneria Informatica S.p.A. | Rome |
| EnginSoft S.p.A. | Trento |
| EnginSoft S.p.A. | |
| ENIA | Isernia |
| Enry’s Island S.p.A. | |
| Enter S.r.l. | |
| Enterprise Consulting S.r.l. | Milan |
| Enterprise S.r.l. | Brescia |
| Enthera S.r.l. | |
| eNTiDi | Brescia |
| Environment Park | Turin |
| Eoliann | Turin |
| Eos Informatica | Brescia |
| Epiave | Treviso |
| EPICODE Education S.r.l. | Rome |
| epiCura S.r.l. | |
| Epiphany S.r.l. | |
| ePrice S.p.A. | Milan |
| Equalis S.r.l. | Milan |
| Ergo Sum S.n.c. | Varese |
| Ergonet S.r.l. | |
| Ericsoft S.r.l. | Rimini |
| Ermes Cyber Security S.p.A. | |
| Ermes S.r.l. | Turin |
| Ernesto | Rome |
| Erptech S.p.A. | Milan |
| ERREsoft S.r.l. | Rome |
| Erresse Service | Sondrio |
| Ersel Banca Privata | Turin |
| ESA Elettronica S.p.A. | Como |
| ESAin S.r.l. | Genoa |
| Esaote S.p.A. | |
| Escogitando S.n.c. | Livorno |