| Alterity Therapeutics | |
| Alternate Studios | Victoria |
| AlterNET Software | New South Wales |
| Altima Interactive | Victoria |
| Altis Consulting | New South Wales |
| Altitude Cowork | Victoria |
| Altrad Consulting Pty Ltd | New South Wales |
| Alyka | Western Australia |
| Alyve | |
| AM-Win | Queensland |
| Amaro | Queensland |
| Amaze Communications | New South Wales |
| AMBA Solutions Pty Ltd | New South Wales |
| Amber Electric Pty Ltd | Victoria |
| Amber Technology | New South Wales |
| Ambire | New South Wales |
| Amblique | New South Wales |
| Amdex | New South Wales |
| AMIRE Digital Marketing Services | New South Wales |
| Amos Scientific Pty Ltd | |
| AMPL Software | New South Wales |
| Ample Tech Australia Pty Ltd | |
| Amplia Therapeutics Ltd | |
| Amplified Engineering Pty Ltd | Western Australia |
| AMPM IT Solutions | |
| Amristar Solutions Pty Ltd | Western Australia |
| AMS Digital | |
| Amused Group Pty Ltd | |
| Amy Who? | South Australia |
| Analysis-one | Queensland |
| Anata Digital | Western Australia |
| Anatomics Pty Ltd | Victoria |
| Anchor Digital | Queensland |
| Anchor Systems | New South Wales |
| Andelo | New South Wales |
| Anderson Morgan | Tasmania |
| ANDHealth Pty Ltd | |
| Andor Information Technology | New South Wales |
| Andromeda Robotics Operations Pty Ltd | Victoria |
| Andzen | Queensland |
| Angry Koala Pty Ltd | New South Wales |
| Anixis | New South Wales |
| Annexa | |
| Anomaly | South Australia |
| Another World Computer Centre | Victoria |
| ANS Communications Pty Ltd | New South Wales |
| Ansarada | New South Wales |
| Anspired Pty Ltd | Queensland |
| Antenna Direct | Western Australia |
| Anteris Technologies | Queensland |