| Queensland Net Pty Ltd | Queensland |
| Queensland University of Technology | Queensland |
| Queensland University of Technology - Information technology and games | Queensland |
| Queensland XR Hub Pty Ltd | Queensland |
| Queness | New South Wales |
| Quick Digital | Victoria |
| Quickclicks | |
| Quickclose Pty Ltd | Victoria |
| QuickOrder | |
| QuietGrowth Pty Ltd | |
| Quinbrook Infrastructure Partners Pty Ltd | |
| Quint Digital | Victoria |
| QuintessenceLabs | Australian Capital Territory |
| QUT Robot Academy | |
| Quuxo Software | New South Wales |
| QVC Solutions Pty Ltd | Queensland |
| Qwilr Pty Ltd | New South Wales |
| R-Group International Pty Ltd | Western Australia |
| R-WEB Ltd | Western Australia |
| R3HYDR8 Pty Ltd | Victoria |
| R6 Digital | |
| Race Oncology | New South Wales |
| Race Oncology Ltd | New South Wales |
| Rack Technologies | New South Wales |
| Rack World Systems | Victoria |
| RackCorp | New South Wales |
| Rad Robotic Accessories | Western Australia |
| Radial Data | New South Wales |
| Radical Systems | |
| Radio Terminal Systems Pty Ltd | Victoria |
| Radiopharm Theranostics | New South Wales |
| Radiopharm Theranostics Ltd | |
| RADLogic Pty Ltd | South Australia |
| Rage Biotech | |
| Raines Internet Solutions | Victoria |
| Rainstick | |
| Raising the Bar | New South Wales |
| Ramcity Pty Ltd | New South Wales |
| Ramp | New South Wales |
| Rampersand | Victoria |
| Random Ramble Pty Ltd | |
| Rank First SEO | Victoria |
| Ranked Ready Websites Services | |
| rankONE | |
| Rankwell Digital Marketing | Victoria |
| Rapid Global | South Australia |
| Rapid Intelligence Pty Ltd | New South Wales |
| RapidFMS Pty Ltd | Victoria |
| RAPT | Queensland |
| Rareiio Pty Ltd | New South Wales |