| LIFT Software | North Holland |
| Lightyear | |
| Lime Networks B.V. | |
| Lindeman B.V. | North Holland |
| Linden-IT | North Holland |
| Lines broadcast B.V. | Gelderland |
| Lingewoud | North Brabant |
| Lingua Franca Consulting B.V. | |
| linkbuildingking.nl | South Holland |
| Linker IT Software B.V. | North Holland |
| Linkit B.V. | Utrecht |
| Linkody | North Holland |
| LinXis B.V. | |
| LioniX B.V. | Overijssel |
| Lipid Knowledge B.V. | |
| Liquit | South Holland |
| Little Robots | |
| Little Rocket | Overijssel |
| Livecom | Utrecht |
| Lizard Global | South Holland |
| LLBLGen Pro | South Holland |
| Local Heroes Worldwide B.V. | |
| LocalFocus | |
| Localhosting | Limburg |
| Localie B.V. | |
| Locamation B.V. | Overijssel |
| Lodder Software | South Holland |
| Logaholic Web Analytics B.V. | North Holland |
| Logi Label | South Holland |
| Logic Vision | South Holland |
| LogicTrade | Overijssel |
| LoginPC | North Holland |
| LogiVert | North Brabant |
| LogSentinel | |
| Loop Robots | South Holland |
| LoopingOne | North Holland |
| Loqus | South Holland |
| LowBudgetPC | Zeeland |
| Loyals Group | Utrecht |
| Loyyo B.V. | |
| LTO Network | |
| LTO Network | North Holland |
| LTO Network | |
| Luci | Limburg |
| LucidWebs | Drenthe |
| Lucrasoft ICT Groep | South Holland |
| Lumicks | North Holland |
| Luminis | Utrecht |
| LUMO Labs | |
| Luna.ai | |