| Notificare | South Holland |
| Notis | South Holland |
| Nou&Off | Gelderland |
| Novabase | Friesland |
| NovaCustom B.V. | |
| Novel-T | Overijssel |
| NOVI | Utrecht |
| Novio Tech Campus | Gelderland |
| Noviosense B.V. | |
| NovoServe | Gelderland |
| Nowatch B.V. | |
| Nowi B.V. | |
| Nowsales – hosting en meer | Utrecht |
| Nowweb | |
| Nox Telecom | |
| NPEX | |
| NPQ Nederland B.V. | North Brabant |
| NSO | South Holland |
| NTF | Gelderland |
| NTH Media B.V. | Gelderland |
| NTrans Technologies B.V. | |
| NTS | North Brabant |
| NTS Computers Technology B.V. | North Brabant |
| Nuffic | South Holland |
| NWB Bank | South Holland |
| NXP Semiconductors | North Brabant |
| O3Spaces B.V. | North Holland |
| Oak & Mie B.V. | |
| Oaky B.V. | |
| OAPEN Foundation | |
| Oberon | North Holland |
| Oberst B.V. | |
| OBI4wan B.V. | North Holland |
| Objective Platform | North Holland |
| Ocdlab | |
| Oceans of Energy | |
| Ockto | |
| Odd.Bot B.V. | |
| Oddity.ai | |
| Odournet Holding B.V. | |
| Office IT | Friesland |
| Offixs | North Holland |
| OGD ict-diensten | South Holland |
| Ohmega Software Platform | South Holland |
| Ohpen | North Holland |
| OJO | |
| Olery B.V. | |
| Olmia Robotics | |
| OLX Group | |
| OMG/Netdirect | |