| XFakt B.V. | North Brabant |
| Xinha | Gelderland |
| Xinvento B.V. | |
| XL Security | |
| XMLBlueprint | Utrecht |
| XOIP B.V. | Gelderland |
| Xolphin.nl | North Holland |
| Xomnia B.V. | North Holland |
| Xperience Internet Solutions | North Holland |
| xQmail.eu | Utrecht |
| XS News B.V. | North Holland |
| XS4ALL | North Holland |
| XSARUS | South Holland |
| Xsdot | North Holland |
| Xsens Technologies B.V. | Overijssel |
| XSserver | North Holland |
| Xtra Creations | South Holland |
| Xtra Digital Agency B.V. | North Brabant |
| XXImo | Utrecht |
| XXL Hosting | Friesland |
| y=f(x) lab | |
| Yabx | |
| Yadi Websolutions | North Holland |
| Yanomo International | North Holland |
| Yapi Kredi Bank Nederland N.V. | |
| yapili B.V. | |
| Yellow Lemon Tree B.V. | Utrecht |
| Yellow Yard B.V. | Groningen |
| Yellowgrape | North Holland |
| Yellowtail Conclusion | |
| YES!Delft | South Holland |
| Yes-Co | Flevoland |
| Yields N.V. | |
| YipYip B.V. | South Holland |
| Ykoon B.V. | South Holland |
| Ylab | Utrecht |
| Ynvolve | Gelderland |
| Yoast B.V. | Gelderland |
| Yoho | |
| Yolknet | North Brabant |
| YOM | |
| Yonego ROI-driven Internet Marketing | North Brabant |
| Yorcom Computers B.V. | South Holland |
| Yorick Peterse | |
| Young_Coders | |
| Younify | Gelderland |
| Your Hosting B.V. | Overijssel |
| Your Next Agency | North Holland |
| YourCampus B.V. | |
| Yource Group | |