| WebMade | South Bohemian |
| WEBMINT, s.r.o. | Prague |
| Webnode AG | South Moravian |
| Webový servis Company, s.r.o. | Prague |
| webProgress, s.r.o. | |
| Webproject | Prague |
| Webrex, s.r.o. | Vysočina |
| WebSite21 | South Bohemian |
| WebStep, s.r.o. | South Moravian |
| WebToo, s.r.o. | |
| Webvalley, s.r.o. | |
| WEDOS Internet, a.s. | Prague |
| WEDOS Internet, a.s. | |
| Wendell electronics, a.s. | Pardubice |
| Wereldo, s.r.o. | |
| Whitesoft, s.r.o. | |
| Windows User Group Czech Republic | Prague |
| WinSCP | |
| WIS Software Group | |
| Wizards CZ, s.r.o. | Pardubice |
| WME Data, a.s. | |
| WolkAbout Technology, s.r.o. | |
| Woltair | |
| WorkLounge Prague, s.r.o. | |
| World from Space, s.r.o. | |
| WSO.cz | Olomouc |
| Wultra, s.r.o. | |
| Xavian Electronics, s.r.o. | Prague |
| Xhosting | Prague |
| Ximilar | South Moravian |
| XIXOIO Ltd | |
| XML Prague | Prague |
| Xone CZ | South Moravian |
| xPORT | |
| Y Soft Corporation | South Moravian |
| Y Soft Corporation, a.s. | |
| yachting.com, s.r.o. | |
| Yako:doma | |
| Ycluster | |
| Yes!Host.ru | Prague |
| Yeye, s.r.o. | |
| Yieldigo, s.r.o. | Prague |
| Yolk Studio, s.r.o. | |
| Yomio, s.r.o. | |
| Your Pass | |
| ysoká škola kreativní komunikace | |
| Z Studio, spol. s r.o. | |
| Z4Soft, Inc. | Prague |
| ZAKA Startup, a.s. | |
| Západočeská univerzita v Plzni | |