| DMS Accelerator | |
| DNS Belgium | Flemish Brabant |
| Dockflow | |
| Dokeos S.P.R.L. | Walloon Brabant |
| Dom4J | Antwerp |
| Domatica | Flemish Brabant |
| Domeinnaam-registreren.net | Antwerp |
| DPG Media Group N.V. | |
| DPO Network Europe | |
| DR Webdesign | West Flanders |
| Drink-IT | Flemish Brabant |
| Drive IT | |
| Drone TechniXX S.P.R.L. | |
| Dropon | |
| Drupal | Antwerp |
| Dualyx N.V. | |
| Duo | West Flanders |
| Duoh! | East Flanders |
| Dust Mobile S.A./N.V. | |
| DVC S.A. | Brussels-Capital |
| DVK Hosting | Flemish Brabant |
| DX-Solutions | |
| Dynamic Software N.V. | Antwerp |
| DZTECH S.P.R.L. | Walloon Brabant |
| e-Health Venture Incubator | |
| E-LAC | Walloon Brabant |
| E-net Business | Namur |
| e-peas | |
| E-Spring Capital | |
| E.DSO | |
| E.R.I.M. S.A. | Walloon Brabant |
| e2e Solutions N.V. | East Flanders |
| EASI S.A. | Walloon Brabant |
| easics N.V. | Flemish Brabant |
| Easy Concept | Hainaut |
| Easy For You | Flemish Brabant |
| Easyhost | East Flanders |
| EasySolution | |
| EatLocal | |
| EBAN | Brussels-Capital |
| Ebusiness.de | Namur |
| eCade S.R.L. | |
| Echo Shop | Brussels-Capital |
| Echosoft S.P.R.L. | |
| Eclips Comm.V | Antwerp |
| Eclipse Foundation AISBL | Brussels-Capital |
| Econocom | Flemish Brabant |
| ECT B.V.B.A. | Antwerp |
| Edebex | |
| Edge.be | Antwerp |