| Ultimate TechnoGraphics, Inc. | Quebec |
| Ultimate Vision Web Design & Consulting | Ontario |
| Ultrahaus Startup Studio | Ontario |
| Ultraspec | |
| Ululab | Quebec |
| Ulysse Biotech | Quebec |
| Umana | Quebec |
| Umanize | |
| Umanologic | |
| Umbrella | Quebec |
| Umso Software, Inc. | British Columbia |
| Un zeste de génie, Inc. | Quebec |
| Una Tech Corporation | British Columbia |
| Unata | Ontario |
| Unbounce | British Columbia |
| Uncanny Owl | Ontario |
| Uncharted Software, Inc. | |
| Undergalaxie.com | Alberta |
| Underlabs | Quebec |
| underlineDESIGN | Alberta |
| Understanding Computers | Ontario |
| UNI Coopération financière | |
| UNI Data, Inc. | New Brunswick |
| UNI Digital Solutions, Inc. | British Columbia |
| Uni-d | Quebec |
| Unibenco, Inc. | Ontario |
| Unicate | Quebec |
| Unicorn Hunt Ltd | |
| Unicorn, Inc. | Ontario |
| Unicycle Digital | British Columbia |
| Unidé Graphique, Inc. | Quebec |
| Unified Computer Intelligence Corporation | Ontario |
| Uniformat II | Quebec |
| Unihedron | Ontario |
| Uniiverse Collaborative Lifestyle, Inc. | Ontario |
| Unik Média | Quebec |
| Unikron | Ontario |
| Unilogik | British Columbia |
| Unilytics Corporation | Ontario |
| Unimark Creative | Alberta |
| Unimatrix Computer Solutions Ltd | |
| Union.Dev Software, Inc. | |
| uniPoint Software, Inc. | Manitoba |
| Uniqair | British Columbia |
| UNiQO Website Design | Ontario |
| Unique Communications, Inc. | Ontario |
| Unique Telecom | Ontario |
| Unique Web Development | British Columbia |
| Uniques Software Corporation | British Columbia |
| Unisearch Associates, Inc. | Ontario |