| Stickyweb Pty Ltd | Victoria |
| Still Not Scared | |
| Stillwell Select Recruitment | South Australia |
| Stimulus | New South Wales |
| Stimulus | |
| Stingrae | New South Wales |
| Stirling Labs Pty Ltd | |
| StitchCraft | New South Wales |
| Stockspot Pty Ltd | New South Wales |
| Stoic VC | |
| Stone & Chalk | New South Wales |
| Stone Digital Pty Ltd | New South Wales |
| Storia Technologies Pty Ltd | |
| Storm Front Technology Pty Ltd | Northern Territory |
| Storm FX Distributions Pty Ltd | New South Wales |
| Storytellers Australia | |
| Straight Path Solutions | Victoria |
| Straight Up One | |
| Straightsell | South Australia |
| Stralia Web | New South Wales |
| Strand7 | New South Wales |
| Strategic Consulting Partnerships | |
| Strategic Directions | Queensland |
| Strategic Group | New South Wales |
| Stratiform | |
| Stream Hub | |
| Streamtime Software Ltd | New South Wales |
| Strike Media | South Australia |
| Stripy Sock | Australian Capital Territory |
| Stronger Digital Co Pty Ltd | |
| StrongPoint Web Design Pty Ltd | Queensland |
| Studio 313 | New South Wales |
| Studio 72 | Victoria |
| Studio Alt | |
| Studio LDN | |
| Studio Twofold Pty Ltd | |
| Studio Web Design | |
| StudioHawk | Victoria |
| Style Atlas | |
| Sublime HQ Pty Ltd | New South Wales |
| Sublime IP Pty Ltd | New South Wales |
| Sublime Text | |
| Success Systems Pty Ltd | Queensland |
| Sumico Net | Queensland |
| Summit Internet | Victoria |
| Summit Software Pty Ltd | Victoria |
| Summit Web Perth | |
| Sun Pharmaceutical Industries Ltd | |
| Sundata Pty Ltd | Queensland |
| SunDrive | |