| Spaceman | New South Wales |
| Spaceship Capital Ltd | New South Wales |
| SPARK Deakin | Victoria |
| Spark Green | Victoria |
| Spark Interact | New South Wales |
| Sparklabs Cultiv8 Pty Ltd | New South Wales |
| SparkLabs Pty Ltd | |
| Sparro Digital Marketing | New South Wales |
| Sparx Systems Pty Ltd | Victoria |
| Spatial Industries Business Association | Australian Capital Territory |
| Spatial Intelligence | New South Wales |
| Spatial Vision | Victoria |
| Spearhead Software | Western Australia |
| Special K Software | Victoria |
| SpectraQEST | South Australia |
| Speedwell | Queensland |
| SpeeDx Pty Ltd | New South Wales |
| Sphere Pty Ltd | New South Wales |
| Spicy Tiger | |
| Spicy Web | |
| Spidersoft | Victoria |
| Spike Innovation | Queensland |
| Spinfluence | New South Wales |
| Spinks Security Pty Ltd | Victoria |
| SpinTel | New South Wales |
| Spirit Telecom Ltd | Victoria |
| Splash | |
| Splashbox | |
| Splat Graphics | |
| SponsoredLinX | |
| Spoon | Western Australia |
| SpoonFeedMe Pty Ltd | |
| Sportsmate | |
| Spot Co-Working | South Australia |
| SpreadsheetGuys.com | New South Wales |
| Spring Web Solutions | |
| Sprint Digital | |
| Sprout Online | Victoria |
| SproutX | Victoria |
| Sqonk Pty Ltd | Victoria |
| Square Peg Capital Pty Ltd | New South Wales |
| Square18 | Western Australia |
| SquareAlpha | Western Australia |
| SQUIDLYdigital | Queensland |
| Squiz | New South Wales |
| SRA Information Technology | |
| SRA Solutions | New South Wales |
| SSW | New South Wales |
| St Vincent’s Institute of Medical Research | |
| St. George Web Design | New South Wales |