| Besturingen.com | South Holland |
| Betronic B.V. | North Holland |
| Betty Blocks | North Holland |
| Bever Automatisering B.V. | Gelderland |
| BeVinced | North Holland |
| Bexter | South Holland |
| bHosted | North Brabant |
| BiCycle B.V. | South Holland |
| BiD Network | North Holland |
| Big Data Expo | |
| BigData Republic | |
| BigSpark B.V. | Gelderland |
| Billink | |
| Billy Grace | North Holland |
| Bilthoven Biologicals | |
| Bimini Biotech B.V. | |
| Bio-Innovations | |
| BioConnection | |
| Biodentify | |
| BioGeneration Ventures | North Holland |
| Biont Research B.V. | |
| BiOraliX B.V. | |
| BiOrion Technologies B.V. | |
| BiosanaPharma B.V. | |
| BioStrand B.V. | |
| Biotalys N.V. | |
| Bird | |
| Bird Electronics B.V. | Gelderland |
| BISIT Engineering | Gelderland |
| BIT | Gelderland |
| Bit Powder | Gelderland |
| Bite | |
| Bitex | North Holland |
| Bitfury Holding B.V. | |
| Bitmap | North Holland |
| Bitonic B.V. | |
| Bits & Parts | |
| Bits Don't Byte | Flevoland |
| Bits&Chips | Gelderland |
| Bits-n-Bones | |
| Bits2Atoms B.V. | |
| Bitsiel | |
| Bitvavo B.V. | |
| BitWise | Overijssel |
| BitWizard B.V. | South Holland |
| Bitz & Pixelz B.V. | North Holland |
| Bizon Software B.V. | North Brabant |
| Bizzdesign | Overijssel |
| BizzSms | North Brabant |
| Bizzy AI B.V. | |