| Bravoure IT | |
| Bread University of Applied Sciences | North Brabant |
| Breathomix | South Holland |
| Bredero Groep | Overijssel |
| Breedband Nederland | Gelderland |
| Breekveldt Automatisering B.V. | Gelderland |
| Bridge Systems B.V. | South Holland |
| BridgeFund B.V. | |
| Bright Answers | South Holland |
| Bright Photonics B.V. | North Brabant |
| BRIGHT Software B.V. | |
| Brilliance Hosting | Friesland |
| Brimit B.V. | |
| Brimore Holding B.V. | |
| Brin | Groningen |
| Bringly B.V. | |
| Brink | South Holland |
| Brinkman.IT | Groningen |
| Broadcast Partners | |
| Brooklyn Venture | Utrecht |
| Brosis.nl | Overijssel |
| Brother International (Nederland) B.V. | North Holland |
| Brxs B.V. | |
| BS Phonefix | |
| BSU Groep B.V. | North Brabant |
| Bttn | |
| BUBTY | South Holland |
| Buckaroo B.V. | Utrecht |
| Buddy Payment B.V. | |
| Budget Computers | South Holland |
| BudgeTronics | North Holland |
| Budgetwebhosting.nl | North Holland |
| Builders | |
| Builders en Performers | Flevoland |
| Bumicom Telecom B.V. | South Holland |
| Bunq | North Holland |
| Bureau Bright | Gelderland |
| Bureau Zigzag | Gelderland |
| Burk Consulting NL B.V. | |
| Buro voor Organisatie & Ontwikkeling | North Brabant |
| Business Angels Netwerken Nederland | North Holland |
| Business Hosting | Overijssel |
| Business IT Rent | Drenthe |
| Business School Netherlands | |
| Business to You B.V. | Utrecht |
| BusinessBase B.V. | Utrecht |
| BUX B.V. | North Holland |
| Buyitdirect | North Holland |
| BVB Media B.V. | North Brabant |
| ByeleX | North Brabant |