| A Must in Every Office B.V. | Overijssel |
| A&A Computer Services | Utrecht |
| A&M Impact Online Creators | Gelderland |
| A. van der Hof Kabelfabriek B.V. | North Brabant |
| A. van Walen Consultancy B.V. | South Holland |
| A.B. software & consultancy B.V. | Overijssel |
| A.H. Automatisering B.V. | |
| A.i Automatisering | |
| A.S. Telecom B.V. | South Holland |
| A/B Smartly | |
| A1 Internet | Friesland |
| Aanmelden Zoekmachines | South Holland |
| AAO Adviesgroep | South Holland |
| Ab Ovo | South Holland |
| ABBO Computers | Gelderland |
| ABC inkt | North Brabant |
| ABC Media | |
| ABC-Software | Gelderland |
| ABCrm | North Brabant |
| Abegaill B.V. | |
| AbeloHost B.V. | |
| AbiSource | Overijssel |
| ABN AMRO Bank N.V. | North Holland |
| About-Blank | North Holland |
| Abstraction Games | North Brabant |
| Academie Verloskunde | |
| Academy to Innovate HR | South Holland |
| Acanthis | South Holland |
| ACC ICT B.V. | Utrecht |
| Accedis | North Holland |
| Accel Club | |
| Accel Club | North Holland |
| Accensys | |
| Accent Interactive B.V. | South Holland |
| Accept | North Brabant |
| Accerion | |
| Access2.IT Group B.V. | |
| Accessibility | Utrecht |
| AccessWeb | South Holland |
| Account Software B.V. | Utrecht |
| Ace | South Holland |
| Ace Multimedia | North Brabant |
| ACE Venture Lab | North Holland |
| AceCardGames | North Holland |
| Acerta Pharma B.V. | |
| Aces Direct B.V. | North Brabant |
| ACES International | Gelderland |
| Aces IT Consultancy & Services | North Brabant |
| AceView | North Brabant |
| ACI B.V. | North Brabant |