| AFAS Software | Utrecht |
| AffinIT | Groningen |
| African Clean Energy B.V. | |
| AfterImage | South Holland |
| AG5 | North Holland |
| Agenda | |
| AGILeBiotics B.V. | |
| AGN | Gelderland |
| AGO Jobs & HR | |
| AgriDirect B.V. | Limburg |
| Agrippa | Limburg |
| Agro Green Systems | |
| Agro IT B.V. | Gelderland |
| AgXeed B.V. | |
| AI in Motion | |
| AI Lab One | South Holland |
| AICN Automatisering | Utrecht |
| Aiden | Utrecht |
| Aiden | |
| Aidence | North Holland |
| Aideon Webdesign | North Holland |
| Aiir Innovations | |
| Aimedis B.V. | |
| Aimforthemoon | North Holland |
| AimValley B.V. | North Holland |
| Aiosyn | Gelderland |
| Airborne | South Holland |
| Airchip B.V. | |
| Airport Telecom | North Holland |
| AIS-Scanology B.V. | North Brabant |
| AISLER B.V. | |
| Aito Touch | North Holland |
| AK IT Solutions | |
| Akara B.V. | Drenthe |
| Akinon | North Holland |
| Akurateco | |
| Albruna | Groningen |
| Alcadis | Utrecht |
| Alex | North Holland |
| Alexander Lichter | |
| Alexion Software | North Holland |
| AlexxDesign | South Holland |
| Alitronika DVS B.V. | Groningen |
| AllartInc | |
| Allero Therapeutics B.V. | |
| AllianceBlock | |
| Allinco Systems | North Brabant |
| Allrent ICT Solutions | Flevoland |
| AllReS | South Holland |
| Allround Automations | Overijssel |