| Ukukhula | |
| Ultimaker B.V. | Utrecht |
| Ultimate Stunts | North Holland |
| Ultimo Software Solutions B.V. | Gelderland |
| UMC Utrecht | |
| Uneedle | |
| UNI Business Centre B.V. | North Holland |
| Unifiedroot | North Holland |
| UNIFORM-Agri | |
| Unify IT | South Holland |
| Unify N.V. | |
| UNIIQ | South Holland |
| uniQure N.V. | North Holland |
| UNIS Group | Friesland |
| Unit 4 Software | South Holland |
| United Courtesy Masters B.V. | |
| United Group B.V. | |
| Universal XS | North Holland |
| Universitair Medisch Centrum Groningen | Groningen |
| Universiteit Leiden | South Holland |
| Universiteit Leiden - Applied Quantum Algorithms | South Holland |
| Universiteit Leiden - Electronics Department | South Holland |
| Universiteit Leiden - Leiden Institute of Advanced Computer Science | South Holland |
| Universiteit Leiden - Mathematical Institute | South Holland |
| Universiteit Leiden - Wiskunde en Natuurwetenschappen | South Holland |
| Universiteit Nyenrode B.V. | Utrecht |
| Universiteit Twente | Overijssel |
| Universiteit Twente - Faculteit Elektrotechniek, Wiskunde en Informatica | Overijssel |
| Universiteit Utrecht | Utrecht |
| Universiteit Utrecht - Departement Informatica | |
| Universiteit Utrecht - Geometry and Quantum Theory | Utrecht |
| Universiteit Utrecht - Mathematical Institute at Utrecht University | Utrecht |
| Universiteit van Amsterdam | North Holland |
| Universiteit van Amsterdam - Institute for Logic, Language and Computation | North Holland |
| Universiteit van Amsterdam - Korteweg-de Vries Institute for Mathematics | North Holland |
| Universiteit voor Humanistiek | |
| University College Roosevelt | Zeeland |
| University of Groningen | Groningen |
| University of Groningen - Computing Science | Groningen |
| University of Groningen - Mathematics | Groningen |
| University of Twente - MESA+ Institute | Overijssel |
| Unlit Studio | |
| Unpluq Technology B.V. | |
| Untied software | North Holland |
| UP learning | Gelderland |
| Up2technology | |
| Uprise | Overijssel |
| Upscene Productions | North Brabant |
| Uptrends B.V. | South Holland |
| Urban Mobility Systems | |