| Velio Ventures B.V. | North Holland |
| Vellekoop & Meesters | Gelderland |
| Velocity Capital | Utrecht |
| Veluwe Partners | |
| VelzArt | Gelderland |
| Vendit B.V. | South Holland |
| Venéco Automatisering, Telecommunicatie & Beveiliging B.V. | South Holland |
| Venema Communicate B.V. | Drenthe |
| Venne Electronics | Limburg |
| Venture Café Rotterdam | |
| Venturerock | North Holland |
| Venturez B.V. | South Holland |
| Verautomation B.V. | North Brabant |
| Verdel ICT & Media | South Holland |
| Vereniging van Universiteiten | South Holland |
| Vergelijk | Overijssel |
| Verifi3D | South Holland |
| Verkaart Automatisering | North Holland |
| Verkerk Webdesign en Service | South Holland |
| Verolme Elektra Systems B.V. | South Holland |
| Verotel Merchant Services B.V. | North Holland |
| Vertigo Games | South Holland |
| Verwey-Jonker Instituut | |
| verything About Computers B.V. | North Brabant |
| Vesper | North Holland |
| Vev | |
| VGM B.V. | Utrecht |
| VHE Industrial Automation B.V. | North Brabant |
| VHIC | South Holland |
| VI Company | |
| VI Technologies | |
| Via Milia | North Brabant |
| Viadesk B.V. | North Holland |
| Vialis B.V. | Utrecht |
| VIB Netwerken | Groningen |
| Vibe Group | North Holland |
| Vibes Consultancy | North Brabant |
| ViCi Consulting | North Brabant |
| ViciniVax | Groningen |
| VICO Therapeutics B.V. | |
| Vict Informatici | North Brabant |
| Vicus eBusiness Solutions B.V. | Utrecht |
| Videtur B.V. | North Brabant |
| Vidicode | South Holland |
| Viewbook B.V. | |
| Viewport.ai | |
| Viggo Smart Hotel | North Holland |
| Vigor & Zest | |
| Vijfhart | Gelderland |
| VIKTOR | |