| 100mega Distribution, s.r.o. | Prague |
| 1C Publishing EU, s.r.o. | Prague |
| 1K Design, s.r.o. | South Bohemian |
| 1st ART Studio s.r.o. | Prague |
| 1st EasySoft | Hradec Králové |
| 1st Webdesign | South Bohemian |
| 2 M STUDIO s.r.o. | Karlovy Vary |
| 24U s.r.o. | Prague |
| 24U, s.r.o. | Prague |
| 24U, s.r.o. | |
| 2N Telekomunikace, a.s. | |
| 3DLabPrint, s.r.o. | |
| 3L Robotics, s.r.o. | |
| 4mobile, s.r.o. | Prague |
| 4Trans Factoring | |
| 74.cz, s.r.o. | Prague |
| A && L soft, s.r.o. | |
| a-net.cz | Prague |
| A.G.A Studio, s.r.o. | South Moravian |
| A.P.S.A., spol. s r.o. | |
| AA Computer, s.r.o. | |
| AAGNETH internet | South Bohemian |
| AB - Credit, a.s. | |
| AB Studio, s.r.o. | Prague |
| AbCheck, s.r.o. | |
| Abeo, s.r.o. | Prague |
| Abonus s.r.o. | Prague |
| ABRA Flexi s.r.o. | Prague |
| ABRA Software a.s. | Prague |
| Abri Design | Zlín |
| ABzone, s.r.o. | Prague |
| Access IT, s.r.o. | Liberec |
| Accomango, s.r.o. | |
| Accord, s.r.o. | Prague |
| ACE Consulting, s.r.o. | |
| Ace IT, s.r.o. | Prague |
| ACEMA Credit Czech, a.s. | |
| Acer Czech Republic | Prague |
| Ackee | Prague |
| Ackee | |
| ACMARK, s.r.o. | South Moravian |
| ACOMWARE, s.r.o. | Prague |
| Acronis | Moravian-Silesian |
| Acropolium | |
| Actinet Informační systémy, s.r.o. | Prague |
| Actinet Informační systémy, s.r.o. | |
| Active 24 s.r.o. | Prague |
| ACTIVE24, s.r.o. | Prague |
| Actnow Ventures | |
| AD Studio, s.r.o. | |