| Atlas Group | |
| ATM Elektro, s.r.o. | Zlín |
| AtomPC | Moravian-Silesian |
| ATS Techplus, s.r.o. | |
| Atweb Consulting, s.r.o. | Prague |
| AUGUR Consulting, s.r.o. | |
| Auksys, s.r.o. | |
| AUSYS informační technologie, s.r.o. | |
| Autoawacs Bohemia s.r.o. | Pardubice |
| Autocont | Moravian-Silesian |
| AV Engineering, a.s. | Zlín |
| Avast Inside Out | |
| Avast Software s.r.o. | Prague |
| Avonet, s.r.o. | Zlín |
| AWS Systems, a.s. | |
| Axe Design Studio | Olomouc |
| AXIMA, s.r.o. | South Moravian |
| AXIS webdesign studio | Zlín |
| Axis, spol. s r.o. | Prague |
| AZ Solutions, s.r.o. | Central Bohemian |
| AŽD Praha, s.r.o. | Prague |
| B:TECH, a.s. | |
| Bach systems, s.r.o. | Olomouc |
| Banka CREDITAS, a.s. | Prague |
| BanksDAILY.com | Prague |
| Barco, s.r.o. | Zlín |
| Bartech | |
| base48, z.s. | |
| Basta digital, s.r.o. | |
| BattShop.eu | Pardubice |
| BCPay | |
| BeBolt | |
| BECO Link, s.r.o. | Pardubice |
| Beep, s.r.o. | Prague |
| BeiT, s.r.o. | |
| BenadeGroup.com | Prague |
| Berghof Systems, s.r.o. | |
| BEST FOR NET, s.r.o. | Prague |
| Bestsite, s.r.o. | |
| BIC Brno spol. s r.o. | |
| Billanc Partners ČR, s.r.o. | |
| BINARGON, s.r.o. | Moravian-Silesian |
| Bioinova, a.s. | |
| BiSigma Česko | |
| Bit CZ Training, s.r.o. | |
| Bit.plus | |
| Bitart | South Moravian |
| BitKinex | Liberec |
| Bitperia | |
| BitServis | |