| Bitt technology ČS, spol. s.r.o. | |
| Biz Data, a.s. | |
| Bleha Jiří | Prague |
| Blockchain Hub Prague | Prague |
| Blocshop, s.r.o. | |
| Blueberry | Prague |
| Blueboard.cz, s.r.o. | Prague |
| BlueGhost | Prague |
| BM Software | South Moravian |
| Bohemian AI Technologies, s.r.o. | Prague |
| Bolt Start Up Development, a.s. | Prague |
| BONDA, s.r.o. | Prague |
| Booking Analytics | |
| Boomerang Communication, s.r.o. | |
| Boost.space | |
| Born Digital, s.r.o. | |
| BossCan ComPrint, s.r.o. | South Moravian |
| Boxart, s.r.o. | |
| BPdata, spol. s r.o. | Prague |
| BRE.cz | Moravian-Silesian |
| Brightify | South Moravian |
| Brigr | |
| Brikk App | |
| Bring Auto, s.r.o. | |
| Brno University of Technology - Faculty of Electrical Engineering and Computing Science | South Moravian |
| BudgetBakers, s.r.o. | Prague |
| Business Communication, s.r.o. | Plzeň |
| Business Factory | |
| ByznysWeb, s.r.o. | |
| C.P.A. CZECH, s.r.o. | Pardubice |
| C3studio.cz | Prague |
| CADware, s.r.o. | Liberec |
| Caligare | Prague |
| CAMEA Technology, a.s. | |
| CAP-NET | |
| Capsa.cz, s.r.o. | Prague |
| Cargonet, s.r.o. | |
| Caroda | |
| Casablanca INT, s.r.o. | Prague |
| Casinvent | |
| CASSINI | Prague |
| CBG Automation, s.r.o. | |
| CCNovasoft, a.s. | |
| ČD - Telematika, a.s. | Prague |
| CDC Data, s.r.o. | South Moravian |
| CDR server, s.r.o. | Prague |
| CEE Bankwatch Network | Prague |
| CEEHACKS | Prague |
| Cenega Czech, s.r.o. | Prague |
| Cenik.cz | Prague |