| UTN Training | West Yorkshire |
| Utopia P2P Ecosystem | |
| UUconstruct | Hertfordshire |
| UWC Atlantic | |
| UWS Creative Computing Technologies | |
| UXBristol | Bristol |
| V-Count | |
| V-Nova | |
| V1 Ltd | Cheshire |
| V1 Technologies Ltd | West Lothian |
| V7 | Greater London |
| V8Media Ltd | Herefordshire |
| VA Technology Ltd | |
| Vaarst | |
| Vaccitech plc | |
| VacV Biotherapeutics (UK) Ltd | |
| Vaioni | |
| VaioRefurbs Ltd | |
| Val-U-Computers | Norfolk |
| Vale Systems | |
| Valen Digital | |
| Valentino's Displays Ltd | |
| Valia | Greater London |
| Valiant Design Ltd | |
| ValiRx plc | |
| Vallibel Finance plc | |
| Value Power Systems Ltd | Shropshire |
| ValueShop | Greater Manchester |
| Vamosa | Glasgow |
| Van der Velde (Service and Support) Ltd | |
| VanderWijk Consultancy | Oxfordshire |
| Vanilla Circus Ltd | |
| Vanilla Electronics | Norfolk |
| VanillaPlus | Kent |
| Vanquis Bank | |
| Vapour Media Ltd | |
| VAR Capital | |
| Varilink Computing Ltd | |
| Varn Media | Wiltshire |
| Vascular Flow Technologies | |
| Vaughan Data Systems | |
| Vault Platform | |
| VaxEquity Ltd | |
| VBX Agency | |
| VC Warehouse Ltd | Cornwall |
| VCG | Greater Manchester |
| Vcodex Ltd | Aberdeen |
| Vecro Tech | Glasgow |
| Vecsoft Ltd | |
| Vecticum | |