| UniCom | Kharkiv |
| Unidata | Kiev |
| Unit Space | Kharkiv |
| UNIT.City | |
| Unity-Bars | Kiev |
| Universe IT | |
| Upmotion.agency | |
| UpScale Ninjas | Kiev |
| Uptech, Inc. | Kiev |
| Upturn | Kiev |
| Uran Company | Zaporizhzhia |
| URich | Zaporizhzhia |
| Usedcisco.ru | Kiev |
| Usoftek | Kiev |
| UTOR | Dnipropetrovsk |
| V. N. Karazin Kharkiv National University | Kharkiv |
| V.E. Lashkaryov Institute of Semiconductor Physics NAS of Ukraine | |
| Vadertek | Kiev |
| Vakoms, LLC | Lviv |
| Valant | Kharkiv |
| Valmax Digital | |
| Valor | Kiev |
| Valor Software | Kharkiv |
| Vcx Library | |
| Vector Software Ltd | Lviv |
| Velton Telecom | Kharkiv |
| Videal | Kharkiv |
| Viktor Goltvyanytsya | Zaporizhzhia |
| VindiTek | Lviv |
| Vinfast | Vinnytsia |
| VinNest ISP | Kiev |
| VIP Quality Software | Odessa |
| Vis-A-Vis | Kiev |
| Visione Interna | Lviv |
| VitGroup | Lviv |
| Viva IT Group | Lviv |
| Viva Software Group | Rivne |
| Vladislav Sokolov | Kiev |
| Vladonai Software | Ternopil |
| Vnet | Dnipropetrovsk |
| Vodafone Ukraine | Kiev |
| Vollna | |
| Volodymyr Muzyka | Zhytomyr |
| Volta One | Kiev |
| Vostok Games | |
| WamiSoftware | Kharkiv |
| WannaBiz | Kiev |
| WarmDevs | |
| Wayforpay | |
| Wayme | |