| Nxtgen Care | Nova Scotia |
| nyclëa | Quebec |
| Nymbus Capital, Inc. | |
| Nymi | Ontario |
| NYN | |
| Nytric Ltd | Ontario |
| NZ Technologies, Inc. | |
| Nzure Tech | |
| N’Ware Technologies | Quebec |
| O Mile-Ex | |
| O'Hara Technologies | Ontario |
| O'Neil Technologies | |
| O-M6 Technologies, Inc. | Quebec |
| O-Netrix Solutions, Inc. | British Columbia |
| O-Possum | |
| O/E Land, Inc. | Quebec |
| O2 Computers | |
| O2 Web | Quebec |
| OA Solutions | British Columbia |
| Oak Bay Softrends | British Columbia |
| Oak Merchant Solutions, Inc. | Ontario |
| Oakbridge Information Solutions | Ontario |
| OARO | |
| Oasis Auto Complete Systems Ltd | Ontario |
| Oasis Computing, Inc. | Ontario |
| OATH Studio | |
| Oatmeal Graphic & Web Design | Nova Scotia |
| Oberland Agriscience | |
| Obie.ai | Ontario |
| Object Computing International, Inc. | Ontario |
| Objectif 8, Inc. | Quebec |
| Objectif Lune, Inc. | Quebec |
| Objectified Software, Inc. | Saskatchewan |
| ObjectSharp | Ontario |
| Objex, Inc. | Ontario |
| Oboulo | |
| OBOX | Quebec |
| Observ | Quebec |
| Observatoire international sur les impacts sociétaux de l'IA et du numérique | |
| Obsidian Strategics, Inc. | Alberta |
| Obvia | |
| Obzerv | Quebec |
| OCAD University | Ontario |
| Occamy Games, Inc. | British Columbia |
| Occasions Affaires | |
| Occupied VR, Inc. | Ontario |
| Ocean Accelerator (CVAP) | |
| Ocean Frontier Institute | Nova Scotia |
| Ocean Legacy | |
| Ocean Networks Canada | |