| Omesa Creative Studio | Ontario |
| Omicron Telesystems, Inc. | Quebec |
| Omid Soft | Ontario |
| Omni Agency | |
| Omni Circuit Boards Ltd | British Columbia |
| Omni Logic Solutions, Inc. | British Columbia |
| Omni Mobile Works, Inc. | |
| Omni Provincial Electronics (Ont) Inc. | Ontario |
| Omni Studios | Saskatchewan |
| Omni Tool Ltd | Ontario |
| OmniaBio, Inc. | |
| OmniBuilder | Ontario |
| Omnico Group | Ontario |
| Omniglobe Solutions | Quebec |
| Omnilogic Online | Saskatchewan |
| Omnilogic Systems, Inc. | Saskatchewan |
| Omnimed | Quebec |
| Omnimedia | |
| Omnimedia, Inc. | Quebec |
| OmniMetrics | British Columbia |
| Omniply | |
| Omniscient Technology, Inc. | Ontario |
| Omnisearch | |
| Omnitech Electronics, Inc. | Nova Scotia |
| Omnitech Innovations, Inc. | Quebec |
| Omnitech Labs | Quebec |
| Omniterm Data Technology Ltd | Ontario |
| Omnius Web Development | |
| Omnivex Corporation | Ontario |
| Omnivision Design | |
| Omnivox Technologies | Quebec |
| Omniware Solutions, Inc. | Ontario |
| OMY Digital Services | |
| On Site Services | Ontario |
| On-Hold Marketing, Inc. | Ontario |
| On-Site Professional Business Services, Inc. | Alberta |
| ON-TRACK Corporate Training | British Columbia |
| OnActuate Consulting, Inc. | |
| OnCall Computer Services | |
| Oncolytics Biotech, Inc. | |
| onConference, Inc. | Ontario |
| Oncopole | |
| Oncoustics | |
| OnDeck Fisheries AI, Inc. | |
| Ondine Biomedical, Inc. | |
| ONDRESYS Technology Solutions | |
| One Day Website | |
| One Design Agency | British Columbia |
| One Eleven | Ontario |
| One Roof Solution | Saskatchewan |