| Space Electronics | Ontario |
| Space Engine Systems | |
| Space Impulse | |
| Space Proof | |
| Space Solution | |
| Space Strategies Consulting Ltd | Ontario |
| Space-O Technologies Canada | Ontario |
| SpaceBridge | |
| Spaceful, Inc. | |
| Spacer Creative | |
| SpaceRyde | |
| Spacestation Labs | |
| Spandrel Interactive, Inc. | |
| Spar Marine Ltd | |
| Spark Angel Investment Network | Ontario |
| Spark Angel Network | Ontario |
| Spark Centre | Ontario |
| Spark Computers | Quebec |
| Spark CRM | British Columbia |
| Spark Innovation | |
| Spark Marketing | |
| SPARK Microsystèmes | |
| SPARK Microsystems International | Quebec |
| Spark New Brunswick | |
| Spark Nova Scotia | |
| Spark RE Technologies, Inc. | British Columbia |
| Spark Training and Coaching Associates, Inc. | Ontario |
| Spark*Advocacy | Ontario |
| Spark+Co | British Columbia |
| Sparked Digital, Inc. | Ontario |
| Sparkgeo | British Columbia |
| SparkIT Solutions | Ontario |
| Sparkjoy Studios | British Columbia |
| Sparkle Marketing | |
| Sparklex Technologies, Inc. | |
| SparkLIT | British Columbia |
| Sparq Systems | |
| Sparrow Acoustics, Inc. | |
| Sparrow Connected, Inc. | |
| Sparrow Studios | Alberta |
| Sparta Capital Ltd | Ontario |
| Sparta Web Solutions | British Columbia |
| Spartan Systems Ltd | New Brunswick |
| Sparton | Ontario |
| Spatial DNA | Ontario |
| Spatial Initiatives Consulting | |
| Spatial Technologies | Alberta |
| SpatialBridge | Ontario |
| SpatiaModels, Inc. | Ontario |
| Speakbox Support, Inc. | British Columbia |