| Spyderspace Group | Ontario |
| SpyNet Solutions, Inc. | Ontario |
| Spyre Infostructure, Inc. | Ontario |
| Spyre Solutions, Inc. | Ontario |
| Spytronic, Inc. | Quebec |
| SQI Diagnostics | |
| SQL CheckUp | Ontario |
| SQL Power Group | Ontario |
| SQL Server Comparison Tool | Ontario |
| SQLSquare, Inc. | Ontario |
| Square Enix Montréal | Quebec |
| Square Peg Communications, Inc. | Ontario |
| Square Zero | Ontario |
| Squareflo | Saskatchewan |
| SquareX Design Studio, Inc. | |
| Squash Labs, Inc. | |
| Squirrel Systems | |
| SR Studio | Ontario |
| SR Technology Services, Inc. | |
| SRA Staffing Solution | Ontario |
| SRFax | British Columbia |
| SRH Software | |
| Srisigma Technologies | |
| SRK Marketing, Inc. | Ontario |
| SRS Informatique | Quebec |
| SS International Consulting Solutions Ltd | Nova Scotia |
| SS Laser Tech Ltd | British Columbia |
| SS Media Co | British Columbia |
| SS&C TIER1 | |
| SSC Security Services Corporation | |
| SSD Data Recovery Toronto | Ontario |
| SSI | British Columbia |
| SSi Canada | Northwest Territories |
| SSI UPS | Quebec |
| SSIMWAVE, Inc. | Ontario |
| SSInfo | |
| SSNI | British Columbia |
| SSP Telecom | Quebec |
| St-Germain Photographie | |
| St-Jean Informatique | Quebec |
| St. Clair College | |
| St. Denis Software Consultants | Ontario |
| St. Francis Xavier University | Nova Scotia |
| St. Francis Xavier University - Computer Science | Nova Scotia |
| St. Jerome's University | |
| St. Lawrence College | |
| St. Martins and District Chamber of Commerce | |
| St. Mary's University | Alberta |
| St. Michael Strategies | Quebec |
| St. Peter’s College | |