| Sparda-Bank München eG | Bavaria |
| Sparda-Bank West eG | North Rhine-Westphalia |
| Sparetech GmbH | |
| Spark Networks SE | Berlin |
| Sparkybit | |
| Sparrow Analytics GmbH | |
| Spath Printware + Service GmbH & Co. KG | |
| Spatz.Digital | Saxony-Anhalt |
| SPAUN Electronic GmbH & Co. KG | Baden-Württemberg |
| SPB Systemorientierte Planungs- und Beratungsgesellschaft mbH | Bremen |
| SPE Systemhaus GmbH | |
| Speakeasy Berlin GmbH | |
| Specter Automation GmbH | |
| Spectra GmbH & Co. KG | Baden-Württemberg |
| Spectra PC & Peripherie GmbH | Baden-Württemberg |
| Spectrm Publishing UG | |
| Spectrum Systementwicklung Microelectronic GmbH | Schleswig-Holstein |
| Speech Code Produktsicherheits GmbH | |
| SPEED Elektronik Vertrieb GmbH | North Rhine-Westphalia |
| Speed Kit | |
| Speed4Trade GmbH | Bavaria |
| Speedlink GmbH | Lower Saxony |
| SpeedPartner GmbH | |
| Speexx | |
| Spegg Kopier- und Informationssysteme GmbH | Hesse |
| SPEKTRA Schwingungstechnik und Akustik GmbH | |
| SphingoTec GmbH | |
| SPHINX Computer Vertriebs GmbH | Bavaria |
| Sphinx Electronics GmbH & Co. KG | Baden-Württemberg |
| Sphinx Worldbiz Ltd | |
| Sphira GmbH | |
| SPICE Group | Berlin |
| spiceCon Management GmbH | |
| SPICO System GmbH | Saxony |
| SPIE | |
| Spiegel Online - Netzwelt | Hamburg |
| SpielFabrique Accelerator Program | North Rhine-Westphalia |
| SPiN AG | Bavaria |
| SpinLab | |
| SpiNNcloud Systems GmbH | |
| Spinner GmbH | Bavaria |
| SPIRIT/21 AG | Baden-Württemberg |
| Spirito GmbH | North Rhine-Westphalia |
| SPIV digital media design | Saxony |
| Splendid Internet GmbH | |
| Split Tech-Solutions GmbH | |
| Splitscreen Studios GmbH | Hamburg |
| spoc.one GmbH | Bavaria |
| Spoke Technologies GmbH | |
| Sport Alliance GmbH | |