| Rotapix Interactive Media | New South Wales |
| Roundhouse | Queensland |
| RoZetta Technology | |
| RPMGlobal Holdings Ltd | |
| RR Apps Pty Ltd | |
| RST Cloud Pty Ltd | New South Wales |
| Ruah Tech Solutions | |
| Rubber Tree Software | New South Wales |
| Rubicon Red | Queensland |
| Rubric | |
| RuleQuest Research Pty Ltd | New South Wales |
| Runway HQ | Victoria |
| Rural Systems | South Australia |
| Rust Jobs Pty Ltd | |
| Rutal Technologies | |
| RxPx | New South Wales |
| Rysen | |
| S1T2 | |
| Saasguru | New South Wales |
| SaaSHub | |
| Saasu | New South Wales |
| SABR Pty Ltd | South Australia |
| SAE University College | |
| Safecoms Cyber Security Pty Ltd | South Australia |
| Safemode Computer Service | New South Wales |
| SafetyCulture | New South Wales |
| Sage Business Solutions Pty Ltd | New South Wales |
| SAGE Group | |
| SAGIPL | |
| Sahan Serasinghe | |
| SAI Global Pty Ltd | |
| Saijo George | Victoria |
| Sales on Sky | |
| Sales Wolfs Australia | |
| Salsa Digital | Victoria |
| Saluda Medical Pty Ltd | New South Wales |
| Sam's Websites | |
| SAMAPOS | |
| Sandglass Enterprises | New South Wales |
| Sanoosa Pty Ltd | Victoria |
| Sapia.ai | Victoria |
| Sapien Technology Pty Ltd | Victoria |
| Sapien Ventures | New South Wales |
| Sarjan Infotech | New South Wales |
| Sash Digital Agency | |
| Satelite and Sound Pty Ltd | South Australia |
| Satisfaction Software Pty Ltd | Queensland |
| SATZ Design | |
| Savage Interactive | Tasmania |
| Saviom Software Pty Ltd | New South Wales |