| Serole Technologies | |
| Servcorp | New South Wales |
| Servers Australia | New South Wales |
| Service Stream Ltd | Victoria |
| ServiceM8 Pty Ltd | Northern Territory |
| Servstra Pty Ltd | Victoria |
| Seveno | Australian Capital Territory |
| Sharesight | New South Wales |
| Shark Digital | |
| Sheridan Institute of Higher Education | |
| Shernet Integrated Services | New South Wales |
| Shift Financial Pty Ltd | New South Wales |
| Shifted Pixels | New South Wales |
| Shine Solutions Group | Victoria |
| Shipfast.agency | |
| Shippit | New South Wales |
| Shiv Technolabs | New South Wales |
| Shivam Technologies | Victoria |
| Shivam Web Design | Victoria |
| Shoalhaven Internet | New South Wales |
| Shopskey | |
| ShopSupport | |
| Shorthand Pty Ltd | Queensland |
| Shotstack Pty Ltd | New South Wales |
| Shout Agency | |
| ShowellTech | |
| Shtudio | |
| Shuttle Marketing Agency | |
| Siaxe | |
| Sibagraphics | Queensland |
| Sibedge | |
| Sidekicker | |
| SideProjectors | |
| Sigma Prime | |
| Signifi Media | |
| Significant | |
| Silicon Cloud International | South Australia |
| Silicon Quantum Computing | New South Wales |
| Silicon Quantum Computing | |
| Silver Communications | Victoria |
| Silver Linings | |
| Simient Pty Ltd | Queensland |
| simpl. Agency | |
| Simple | |
| Simple KYC Pty Ltd | New South Wales |
| Simple Net Australia Pty Ltd | Victoria |
| Simple Pages | Queensland |
| Simple Retail | New South Wales |
| SimpleDiagrams | Tasmania |
| Simply Savvy Web | Western Australia |