| Specialist Retail Systems B.V. | Flevoland |
| SpeechCloud | |
| Spel-Maker | North Holland |
| Spice Productions | Limburg |
| Spil Games | North Holland |
| Spinware Solutions B.V. | South Holland |
| Spirit IT B.V. | North Brabant |
| Spirit Solutions B.V. | Limburg |
| Spitters | North Brabant |
| Sportmonks | Overijssel |
| Spot Software B.V. | North Holland |
| Spotler | South Holland |
| Spotr | |
| Spotzer | North Holland |
| Spraakmaker Telecom | Gelderland |
| Sprague Europe B.V. | Gelderland |
| Sprigs | North Holland |
| SpringTeam B.V. | Utrecht |
| Sprinque B.V. | |
| SPRINT Robotics | |
| spriteCloud B.V. | |
| Spruit Digital | |
| Spryng B.V. | North Holland |
| SPS Holding B.V. | South Holland |
| SPS International | Gelderland |
| SQR.nl | North Holland |
| Squads B.V. | |
| Square One Solutions B.V. | North Holland |
| SquareDesign | Gelderland |
| Squeezely | |
| SRON | Utrecht |
| SSP Simple Soft Products | Flevoland |
| Stackscale B.V. | |
| Stallion Automatisering B.V. | North Brabant |
| StanDev | |
| Starodub B.V. | |
| Starred | North Holland |
| Start People | |
| StartDock | |
| Starteenwinkel | |
| StartHub Wageningen | Gelderland |
| StartLife | Gelderland |
| Startpage | South Holland |
| Startpunt Internetn | Gelderland |
| Startup Almere | |
| Startup Amsterdam | North Holland |
| Startup Foundation | |
| Startup in Residence | North Holland |
| StartUp Scan | North Holland |
| Startup Thermometer | Friesland |