| Startup Twente | Overijssel |
| Startup Village | |
| Startup Village | |
| Startupboot | |
| Startupbootcamp Bold Action | North Holland |
| Startupbootcamp Sustainability | North Holland |
| StartupTalks | |
| Stat.me B.V. | |
| Staten Biotechnology B.V. | |
| Steam Data Suite | North Holland |
| Stebis B.V. | Gelderland |
| Steens & Partners | |
| Stellantis N.V. | |
| Stepco B.V. | Limburg |
| Stershop B.V. | North Brabant |
| STH Automatisering | |
| Stichting Pedagogisch Onderwijs | |
| Stichting Publieke Zaken | North Holland |
| Stichting Telecom | Utrecht |
| Stichting Wireless Leiden | South Holland |
| Stillio B.V. | |
| Stimmt Digital B.V. | North Holland |
| Stipt-IT | |
| Stoneart B.V. | Groningen |
| Straight IT | |
| Straight Systems | Gelderland |
| StraightFire Studios | |
| Strangelove | |
| Striktly Business Software B.V. | |
| STRM | South Holland |
| Strypes EOOD | North Brabant |
| STS Automatisering | Groningen |
| Studio Bosgra | |
| Studio Carpe Diem | |
| Studio Projectie B.V. | South Holland |
| Studio Van Pelt | North Holland |
| Studio-Max | Utrecht |
| STUDIO.WHY | Gelderland |
| Studio16 | Utrecht |
| Studocu | North Holland |
| Studyportals Bachelors | |
| Studytube B.V. | |
| Stuvia International B.V. | |
| SubsidyCloud | South Holland |
| Sudus Internet | Utrecht |
| Sulfateq B.V. | |
| Sumatra Software B.V. | South Holland |
| Superior B.V. | North Brabant |
| SuperOffice Benelux B.V. | North Brabant |
| SuperSaaS | North Holland |