| Komix | Prague |
| Kontent.ai | |
| Kontrolujeme, s.r.o. | |
| Koopeo Ventures | |
| KoPlac | |
| Korowatt, s.r.o. | |
| Kostax, s.r.o. | South Bohemian |
| Kostečka GROUP spol. s r.o. | |
| KP-SYS, spol. s r.o. | Pardubice |
| KROB software, s.r.o. | |
| Krofian CZ, s.r.o. | Liberec |
| Krup, s.r.o. | Prague |
| KSH-Data | Pardubice |
| KTK SOFTWARE, s.r.o. | Liberec |
| Kurent, s.r.o. | |
| Kvapem.cz | Prague |
| Lama plus | Moravian-Silesian |
| LAMIREL PCB Europe, s.r.o. | Hradec Králové |
| LangSoft, s.r.o. | |
| LazNet, s.r.o. | Prague |
| Leadspicker | |
| LEC, s.r.o. | Prague |
| Lednet, s.r.o. | |
| Legrand, s.r.o. | Prague |
| LEITNER Slovensko, s.r.o. | |
| Level, s.r.o. | |
| LICA CZECH, s.r.o. | Central Bohemian |
| Lighthouse Ventures | |
| Limasoft, s.r.o. | |
| Linhart Marketing | South Moravian |
| LinuxBox.cz | Moravian-Silesian |
| LinuxEXPRES | South Moravian |
| Lira IS, s.r.o. | Prague |
| LitFin Capital, a.s. | |
| Livesport s.r.o. | Prague |
| Livesport, s.r.o. | |
| Liware, s.r.o. | |
| LK-Hardware, s.r.o. | |
| LLP Group | Prague |
| Localazy | |
| Locus Workspace, s.r.o. | |
| Logio | |
| Look AI Ventures SICAV, a.s. | |
| Lukáš Barták | Prague |
| Lukáš Foldina | Moravian-Silesian |
| Luky's Soft | Prague |
| Lunaria, s.r.o. | |
| Lundegaard Slovensko s. r. o. | Prague |
| Lupa.cz | Prague |
| LutherOne plc | |