| Microcomp, spol. s.r.o. | |
| Microsoft Czech Republic | Prague |
| Microton, a.s. | Zlín |
| Mifomobil | |
| Milk Computers, s.r.o. | Prague |
| Milsoft, a.s. | |
| Mingle Games | |
| Minion Interactive, s.r.o. | Liberec |
| MiradiX, s.r.o. | South Bohemian |
| MiriSys Software, s.r.o. | |
| Mironet | Prague |
| MITON CZ, s.r.o. | Liberec |
| MK Consult, v.o.s. | Ústí nad Labem |
| MOBILCOMP, s.r.o. | Moravian-Silesian |
| MobileShop.eu | |
| Mockuuups Studio | |
| Mokry Systems | South Moravian |
| Mokry Systems, s.r.o. | South Moravian |
| Mondo cs, spol. s r.o. | Vysočina |
| MONET+, a.s. | Zlín |
| MONETA Money Bank, a.s. | Prague |
| Money S3 | South Moravian |
| Monika Novotna | Prague |
| Mont-Tech s.r.o. | |
| MoroSystems | Prague |
| Motionlab | |
| Motobit.com | Central Bohemian |
| Movinio App, s.r.o. | |
| MP-Soft, a.s. | |
| MPalán, s.r.o. | South Moravian |
| MRP-Informatics, spol. s r.o. | Zlín |
| MSD, spol. s r.o. | South Moravian |
| mSpy | |
| MSTECH | |
| MultiMedia SoftWare | Hradec Králové |
| Mutumutu.cz | |
| MyDreams | Central Bohemian |
| Myia Systems, s.r.o. | |
| N&N, spol. s r. o. | |
| Nangu.TV | |
| Nanorobots Research Center | |
| Napoleon Games, s.r.o. | Prague |
| Náš NET, s.r.o. | Central Bohemian |
| Náš-Net.org | Prague |
| Nativa Enterprise, s.r.o. | Moravian-Silesian |
| Navigo3 | South Moravian |
| Navisat, s.r.o. | Prague |
| Navisys, s.r.o. | |
| NECOSS, s.r.o. | South Bohemian |
| NejŘemeslníci.cz | |