| Optifor, s.r.o. | Prague |
| Optima Recruitment Europe, s.r.o. | |
| Optimalizace a SEO | Prague |
| OptimSys, s.r.o. | South Moravian |
| Optimus | South Moravian |
| ORBIT, s.r.o. | |
| Organizační kancelář Znojmo, spol. s.r.o. | |
| Orionet | |
| Orlovanet.cz | |
| Oscar Senior, s.r.o. | |
| P.V.A. systems, s r.o. | |
| PalmApp | |
| PAMA, spol. s r.o. | Zlín |
| Panevropská univerzita | |
| Pantek (CS), s.r.o. | Hradec Králové |
| Paralelní Polis | |
| PartnerCIS, s.r.o. | Zlín |
| Passengera | |
| PBS Group, a.s. | |
| PC - Servis Kozák | Central Bohemian |
| PC HELP, a.s. | Vysočina |
| pc-ambulance | Prague |
| PC-Progress s.r.o. | Prague |
| PCKURZY.cz | Prague |
| PCV Computers, s.r.o. | |
| PCWorld.cz | Prague |
| Peccala | |
| PeckaDesign | South Moravian |
| Peering.cz | Prague |
| Penta Investments, s.r.o. | |
| Pernica IT Solutions, s.r.o. | South Moravian |
| Perseus, s.r.o. | Central Bohemian |
| Persoo, s.r.o. | Prague |
| PetaMem, s.r.o. | Prague |
| Petr Kovář | Prague |
| Petr Pavlík Counselling | Prague |
| Petrushev Capital | |
| Photoneo, s.r.o. | |
| Photopea | |
| Phrase | |
| PictView | Prague |
| Pipette & Chart, s.r.o. | |
| PIPNI, s.r.o. | Prague |
| Pixelhouse, s.r.o. | South Moravian |
| Pixelmate | Prague |
| Plan4all z.s. | |
| Plus4U Net, a.s. | |
| PM Consulting | |
| Poda, a.s. | Moravian-Silesian |
| PodporaObchodu | Hradec Králové |