| Empion GmbH | |
| Employer Branding. Tech | |
| Empolis GmbH | Rhineland-Palatinate |
| EMS Press | |
| Emtec GmbH | Bavaria |
| EmTec Innovative Software | Bavaria |
| Emteria GmbH | North Rhine-Westphalia |
| eMundo GmbH | Bavaria |
| ena Webstudio | Brandenburg |
| Enable Security GmbH | |
| enaDyne GmbH | |
| ENAiKOON GmbH | Berlin |
| EnBW New Ventures GmbH | |
| encad consulting GmbH | |
| Encentive GmbH | |
| EnCo Software GmbH | |
| encourageventures e. V. | |
| encoway GmbH | |
| Encryption-Software.de | Bavaria |
| Encurio GmbH | |
| Endel | |
| endios GmbH | |
| ENDOSMART Gesellschaft für Medizintechnik mbH | |
| Enercon GmbH | Lower Saxony |
| Energienetze Mittelrhein GmbH & Co. KG | |
| Energieparking | Rhineland-Palatinate |
| Energy Robotics | |
| Energy Robotics GmbH | |
| ENERGY4U GmbH | Baden-Württemberg |
| enforce AG | Berlin |
| Engage Media | North Rhine-Westphalia |
| Engel & Co. GmbH | Thuringia |
| Engel Elektromobile GmbH | |
| Engelking Elektronik GmbH | Baden-Württemberg |
| Engelmann Software GmbH | North Rhine-Westphalia |
| Engels & Fagel GmbH | North Rhine-Westphalia |
| EngFlow GmbH | |
| Engineering Consulting & Solutions GmbH | Bavaria |
| EngInSite | North Rhine-Westphalia |
| engram GmbH | Bremen |
| Enit – eine Marke der Böhme & Weihs GmbH | |
| EnjoyVenture GmbH | |
| Enlinvar GmbH | Berlin |
| Enmacc GmbH | |
| Enna Systems GmbH | |
| Enneagramm Software | Hesse |
| Ennit AG | Schleswig-Holstein |
| Enomic GmbH & Co. KG | Baden-Württemberg |
| Enorm GmbH | Bavaria |
| enosys Recycling GmbH | Mecklenburg-Vorpommern |