| Enote GmbH | |
| Enotech GmbH | |
| Enpal B.V. | |
| Enscape GmbH | |
| Ensys GmbH | |
| Enteas GmbH | |
| Entegra Eyrich + Appel GmbH | Baden-Württemberg |
| Entero AG | Hesse |
| Enterra Beratung und Vertrieb GmbH | Baden-Württemberg |
| EnterTrain Software GmbH | Baden-Württemberg |
| Entiretec AG | |
| Entrecode GmbH | |
| Entrepreneur University | Hesse |
| Entrepreneurs Club Cologne | North Rhine-Westphalia |
| Entrepreneurship Zentrum Witten gGmbH | |
| Entwickler.de | Hesse |
| ENUM-Center | Hesse |
| Envalior | |
| Envel Informationssysteme GmbH | Bavaria |
| Envers AG | North Rhine-Westphalia |
| Envia TEL GmbH | Saxony |
| Envidatec GmbH | Hamburg |
| Enviria Energy Holding GmbH | |
| Envise Werbeagentur | Lower Saxony |
| ENVISYS GmbH & Co. KG | Thuringia |
| Envoria | |
| Enway GmbH | |
| eobiont GmbH | |
| Eolane SysCom GmbH | |
| EOMAP GmbH & Co. KG | |
| EOS GmbH | Bavaria |
| EPAG.de | North Rhine-Westphalia |
| EPAK GmbH | |
| epekworks GmbH | |
| ePhilos AG | Bremen |
| Epicenter Media GmbH | |
| epicrails IT consulting | |
| Epictet AG | Bavaria |
| Epigenomics AG | |
| Epilot | North Rhine-Westphalia |
| epis Automation GmbH & Co. KG | Baden-Württemberg |
| Epitype GmbH | |
| EPLAN GmbH & Co. KG | |
| ePlanIT | Schleswig-Holstein |
| EPOS CAT GmbH | Bavaria |
| EPOS GmbH | Bremen |
| epos Systemhaus GmbH | |
| Eppendorf SE | |
| EppleMultimedia GmbH | Baden-Württemberg |
| Epson Deutschland GmbH | North Rhine-Westphalia |