| Robin | Gelderland |
| Robin Mobile | |
| Robin Radar | South Holland |
| RoboMiri | South Holland |
| Robota Softwarehouse B.V. | North Holland |
| RobotWise | |
| Robovision B.V. | |
| RobSoftware | Overijssel |
| RocketX | |
| Rockstart | North Holland |
| Rockstart | North Holland |
| Rocom B.V. | Limburg |
| Rocsys | South Holland |
| Rodeo | |
| ROES IT B.V. | North Brabant |
| Roessingh Research and Development | Overijssel |
| Roger B.V. | |
| Roho Products | Overijssel |
| Rolls Software B.V. | North Brabant |
| Romex B.V. | Utrecht |
| RONIS | Groningen |
| RoodMicrotec N.V. | |
| Rotterdam Innovation City | South Holland |
| Rotterdam Open Data | South Holland |
| Roundabout | North Holland |
| Route4Gas B.V. | |
| Routershop.nl | Utrecht |
| RoutIT B.V. | |
| Rovecom | Drenthe |
| Royal NLR – Netherlands Aerospace Centre | |
| RoyaleHosting | |
| RPO Automatisering B.V. | South Holland |
| RS Technics B.V. | Drenthe |
| RTP Software | North Holland |
| Ruitech Solutions B.V. | |
| S for Software | Gelderland |
| S&S Online Marketing | Flevoland |
| S-Inside Design & Development | Limburg |
| S-Trade | North Brabant |
| S4 Energy | South Holland |
| S4 Energy B.V. | |
| Saas Now B.V. | South Holland |
| SaaSplaza International | North Holland |
| Sab Training B.V. | South Holland |
| SabanWorld | South Holland |
| Sabra Systems B.V. | |
| Sageon B.V. | |
| Saia Agrobotics B.V. | |
| SailRouter | South Holland |
| Sairopa B.V. | |