| SALAR Software | North Brabant |
| Salarise | |
| Sales Rocks | South Holland |
| SalesManager Software | North Holland |
| Salland Engineering (Europe) B.V. | Overijssel |
| Salonized B.V. | |
| Samotics | South Holland |
| Samotics | South Holland |
| Sanaga Ventures | North Holland |
| SanBio B.V. | |
| Sandra Kingma Grafische Vormgeving | Gelderland |
| Sanicom Telecommunicatie B.V. | South Holland |
| Santhos | Drenthe |
| Sapenta | South Holland |
| Sapreme Technologies | Utrecht |
| Sark7 | South Holland |
| Satellietdiscount.com | Limburg |
| Satelligence | Utrecht |
| Satsearch B.V. | |
| Satys B.V. | |
| Saxion University of Applied Sciences | Overijssel |
| Saylus | Gelderland |
| SBeffect | Zeeland |
| Scala Solutions | |
| Scaldis ICT B.V. | |
| Scale Cities Alliance | North Holland |
| ScaleX Ventures | North Holland |
| Scamander | Utrecht |
| ScanmarQED | Utrecht |
| Scenic Biotech | North Holland |
| SchedJoules | |
| SchiozziWeb | |
| Schiphol Telematics B.V. | North Holland |
| Science Park Amsterdam | North Holland |
| Scientific Volume Imaging | North Holland |
| Scitodate B.V. | |
| Scoopexpert B.V. | |
| SCOPE marketing technology B.V. | North Holland |
| Scopic B.V. | North Holland |
| SCOS | North Holland |
| ScraperBox | North Holland |
| ScreenCheck B.V. | South Holland |
| ScreenPoint Medical B.V. | |
| Scriptware | North Holland |
| SDB Groep | |
| SDB Java | South Holland |
| SDC B.V. | Gelderland |
| SDIM | North Holland |
| SDO Hogeschool | |
| Seal It Services B.V. | South Holland |